Single cell gene expression profiling of the response of the dorsal motor nucleus of the vagus neurons to remote ischemic preconditioning [DMV_RIPC_RT-PCR]

We developed a mesoscale single cell transcriptomics data from hundreds of the dorsal motor nucleus of the vagus (DMV) neurons under homeostasis and following physiological perturbations. Our results revealed that homeostatic DMV neuronal states can be organized into distinguishable input-output signal processing units. Remote ischemic preconditioning induced a distinctive shift in the neuronal states towards diminishing the role of inhibitory inputs. Male sprague dawley rats were subjected to remote ischemia preconditioning (rIPC) through ligation of bilateral femoral arteries or sham operation incluing ketamine/xylazine anesthesia and surgical exposure without ligation. n=2-3 biological replicates. All samples were collected 2.5 hours after referfusion.