Genome-wide expression profiling of 4T1 cells to identify genes that are regulated by Id1 and Id3 in breast cancer. Genome-wide expression profiling of 4T1 cells to identify genes that are regulated by Id1 and Id3 in breast cancer

By combining RNAi-mediated knockdown of Id1 and Id3 in an aggressive mouse breast cancer cell line (4T1 cells) with genome-wide expression profiling, we identified several new Id target genes and novel pathways regulated by Id. Overall design: Gene expression profiling was performed on Control (C) and Id1 and Id3 depleted 4T1 cells. The gene expression profiles of four independent replicates (R1, R2, R3 and R4 ± doxycycline treatment) were compared by microarray analysis