Global protein profiling of HaCaT cells following 30-minute heat stimulation at 44°C

This proteomic study employed high-throughput liquid chromatography-tandem mass spectrometry (LC-MS/MS) to systematically analyze the dynamic changes in the global proteome of human immortalized keratinocytes (HaCaT) following 30 minutes of hyperthermia stimulation at 44°C.HaCaT cells were subjected to either 44°C hyperthermia stimulation for 30 minutes or a 37°C water bath treatment for 30 minutes as the control group. Proteins were extracted 4 hours post-treatment for subsequent analysis, with three biological replicates performed for each condition. The dataset includes:
hacat44-1, hacat44-2, hacat44-3: Three biological replicates of the 44°C hyperthermia-treated group.
hacat37-1, hacat37-2, hacat37-3: Three biological replicates of the 37°C control group.
In this dataset:
FoldChange indicates the differential expression level:
FoldChange > 1: The protein expression is upregulated after hyperthermia stimulation.
FoldChange < 1: The protein expression is downregulated after hyperthermia stimulation.
The closer the value is to 1, the smaller the change; the farther from 1, the greater the difference.
logFC (log2 FoldChange) represents the log2-transformed fold change:
logFC > 0: The protein expression is upregulated after hyperthermia stimulation.
logFC < 0: The protein expression is downregulated after hyperthermia stimulation.
The closer the value is to 0, the smaller the change; the farther from 0, the greater the difference.