Expression of a corA-lacZ transcriptional fusion in Salmonella

A corA-lacZ transcriptional fusion was shown to be activated by RpoS when Salmonella is grown to stationary phase in LB rich medium (Metaane et al.  2022)
Here, expression of the corA-lacZ fusion was evaluated in Salmonella wild-type strain and rpoS mutant grown to stationary phase in LB medium containing different concentrations of Mg and in minimal medium M63 starved or not for Mg. RpoS was required fort optimal expression of the fusion in these three environmental conditions and level of expression was slightly higher when the extracellular magnesium concentration was low.
Metaane S, Monteil V, Ayrault S, Bordier L, Levi-Meyreuis C, Norel F. The stress sigma factor sigmaS/RpoS counteracts Fur repression of genes involved in iron and manganese metabolism and modulates the ionome of Salmonella enterica serovar Typhimurium. PloS one 2022, 17(3):e0265511.
This work was supported by the French National Research Agency (ANR-19-CE44-0005-01, PERIOMET project).
See also:
Metaane S, Monteil V, Douché T, Giai Gianetto Q, Matondo M, Maufrais C, Norel F. Loss of CorA, the primary magnesium transporter of Salmonella, is alleviated by MgtA and PhoP-dependent compensatory mechanisms. PloS one 2023, 18(9):e0291736.
NOREL, MONTEIL, & METAANE. (2023). Towards new elements involved in magnesium and cobalt trafficking in Salmonella serovar Typhimurium. Zenodo. https://doi.org/10.5281/zenodo.8086417
NOREL, METAANE, & MONTEIL. (2023). Detection of physical interactions between the magnesium transporter CorA and other Cor proteins using the bacterial two hybrid system (BACTH). Zenodo. https://doi.org/10.5281/zenodo.7994619
NOREL Francoise, MONTEIL Veronique, DOUCHE Thibaut, & MATONDO Mariette. (2023). Global effects of deletions of the sitABCD, mntH, cbiMNQO and corA genes, encoding transporters for manganese, cobalt and magnesium on protein abundance in Salmonella enterica serovar Typhimurium grown to stationary phase in LB. [Data set]. Zenodo. https://doi.org/10.5281/zenodo.8279780