Stable isotope data from reef fish collected at natural reefs sites in the northern Gulf of Mexico from November 2010 – August 2011.

Approximately 50 grams of white muscle tissue was removed from reef fish to perform stable isotope analysis. Muscle tissue samples were dried at 60°C for at least 24 hours, and then ground into a fine powder with a glass or agate mortar and pestle. Mortars and pestles were cleaned with 2% nitric acid and rinsed with Milli-Q water between samples to prevent cross contamination. Ground samples containing between 0.2 and 0.5 g of pulverized dried tissue were placed in sterile plastic centrifuge tubes and shipped to a contract laboratory to perform analysis of d13C, d15N, and d34S with a Europa Scientific GSL/Geo 20-20 stable isotope ratio-mass spectrometer (SIR-MS). The isotopic ratio of a sample relative to the ratio in a standard was reported as a delta value.为开展稳定同位素分析（stable isotope analysis），从珊瑚礁鱼类中获取约50克白肌组织。将采集的肌组织样本置于60℃环境下烘干至少24小时，随后使用玻璃或玛瑙研钵与研杵将其研磨为细粉。为防止样本间发生交叉污染，每处理完一个样本后，均需用2%硝酸清洗研钵与研杵，再用密理博（Milli-Q）超纯水冲洗干净。称取0.2至0.5克研磨后的干燥组织粉末，置于无菌塑料离心管中，随后寄送至签约实验室，使用Europa Scientific GSL/Geo 20-20型稳定同位素比值质谱仪（stable isotope ratio-mass spectrometer, SIR-MS）对样本的δ¹³C、δ¹⁵N及δ³⁴S进行分析。样本的同位素比值相较于标准参考物质的比值，以δ值（delta value）的形式予以报告。