Development of microRNA expression in exosomes derived from lncRNA HOTTIP overexpressed FaDu cells. Development of microRNA expression in exosomes derived from lncRNA HOTTIP overexpressed FaDu cells

Purpose: to detect the expression differences of miRNAs in exosomes derived from HOTTIP-overexpressed and NC FaDu cells Methods: Total RNA containing small RNA was extracted from oe-HOTTIP FaDu-derived exosomes. Use Agilent miRNA array for miRNA analysis. Each slide of the Agilent array is designed as eight identical arrays (8×60K format), and each array contains probes for detecting 2549 human mature miRNAs from miRBase R21.0. Each miRNA was repeatedly detected by the probe 30 times. The array also contains 2164 Agilent control probes. GeneSpring software V13 (Agilent) was used to analyze the miRNA array data for data aggregation, standardization and quality control. Perform the default 90th percentile normalization method for date preprocessing. To select differentially expressed genes, we used thresholds of ≥2 and ≤−2 fold change, and the Benjamini-Hochberg corrected p vlaue was 0.05. Results: The expression of 5000 miRNAs were tested and 257 miRNAs were significantly down-regulated. Conclusion: 257 down-regulated miRNAs were candidates for HOTTIP-associated ceRNA network Overall design: Examination of the expression differences of mircoRNAs in exosomes derived from HOTTIP-overexpressed and NC FaDu cells