Proportions of sporocyte and mature cyst stages of Pneumocystis carinii.

Whole organism population was isolated from the lungs of immunosuppressed animals as previously described [27]. Sporocyte and mature cyst stages were counted from RAL-555-stained smears according to the number of nuclei in each life cycle stage. Percentages of sporocyte and cystic stages with N nuclei in the fraction of sporocytes and mature cysts were given as mean of three independent experiments (n = 70, n = 55, n = 50). N = number of nuclei per fungal cell. SD = standard deviation.(a)Early sporocytes.(b)Sum of intermediate sporocytes with 2, 3 and 4 nuclei.(c)Sum of late sporocytes and mature cysts with 5, 6, 7 and 8 nuclei.

如前所述[27]，本研究从免疫抑制动物的肺部分离得到整个有机体种群。依据各生命周期阶段核数量的统计，对RAL-555染色涂片中的子囊孢子阶段及成熟子囊阶段进行计数。子囊孢子与成熟子囊阶段中具有N个核的百分比数据以三次独立实验的平均值给出（n=70，n=55，n=50）。N代表每个真菌细胞中的核数。SD代表标准偏差。（a）早期子囊孢子。（b）具有2、3和4个核的中间子囊孢子的总和。（c）具有5、6、7和8个核的晚期子囊孢子与成熟子囊的总和。