Differences in bone marrow serum proteins in acute myeloid leukemia patients and normal controls

we evaluated cytokine profiling of bone marrow serum samples in AML patients and healthy controls. Protein expression profiling of serum from 9 AML patients and 5 healthy controls was obtained using biotinylated antibody chip. Total 507 cytokines and growth factors were analyzed. Compared with healthy people, AML patients expressed 31 signature proteins, among which, 27 were found significantly higher expressed and 4 proteins were lower. 9 AML patients and 5 healthy controls were enrolled in this study, Whole blood was collected in vacutainer serum tubes, and then 507 serum proteins were analyzed simultaneously using an antibody-based array.