Bioelectrical impedance analysis for an endangered desert fish—Data

These data were used to examine the effectiveness of a non-lethal tool (Bioelectrical Impedance Analysis, "BIA") to estimate the physiological condition of endangered and threatened fishes in the Colorado River Basin. We conducted laboratory trials using hatchery-raised Humpback Chub and Bonytail and wild-captured Roundtail Chub, where fish were subjected to different feeding trials to elucidate a response in physiological condition and different temperature treatments to approximate field conditions. At the end of each 6-week trial fish were removed from tanks, lateral and dorsal measurements of BIA were taken, and fish were sacrificed for proximate composition analysis (lipid, protein, water, ash, dry mass, energy density). We developed models that evaluated the effectiveness of BIA alone vs. standard morphological condition-based measurements taken in the field (e.g., using total length and wet mass). We conducted a simulation experiment to increase the observation error around morphological wet mass measurements to determine the crititcal error at which BIA becomes a more important predictor of proximate composition than condition factor. In addition, we tested the influence of different BIA temperature conversion factors (20 C, 15 C, none) for use in Humpback Chub GLMM models. We also conducted an experiment to determine mortality rates when standard field methods or BIA is used to estimate fish condition. Detailed methods and results are reported in the manuscript associated with this data publication. These data are associated with the journal manuscript:Dibble, K.L., Yard, M.D., Ward, D.L., and Yackulic, C.B., 2017, Does bioelectrical impedance analysis accurately estimate the physiological condition of threatened and endangered desert fish species?: Transactions of the American Fisheries Society v. (online), https://doi.org/10.1080/00028487.2017.1302993..

本数据集用于评估一种非致死工具——生物电阻抗分析（Bioelectrical Impedance Analysis, BIA）对科罗拉多河流域濒危与受胁鱼类生理状态的估算效能。本研究利用孵化场繁育的驼背鲮（Humpback Chub）、骨尾白鲑（Bonytail）以及野外捕获的圆尾鲮（Roundtail Chub）开展室内试验：设置不同投喂梯度以解析鱼类生理状态的响应变化，并设置不同温度梯度以模拟野外环境条件。每轮6周的试验周期结束后，将试验鱼从养殖缸中取出，采集体侧与背部的生物电阻抗数据，随后处死试验鱼以开展常规营养成分分析，检测指标包括脂质、蛋白质、水分、灰分、干重与能量密度。本研究构建了模型，对比评估了单独使用生物电阻抗分析，与野外常规基于形态学状态的测量方法（例如利用鱼体全长与湿重）的估算效能。本研究开展了模拟试验，通过增大形态学湿重测量的观测误差，以确定临界误差阈值：当观测误差达到该阈值时，生物电阻抗分析对常规营养成分的预测能力将优于条件因子法。此外，本研究针对驼背鲮的广义线性混合模型（GLMM, Generalized Linear Mixed Models），测试了不同生物电阻抗分析温度校正因子（20℃、15℃以及无校正）的影响效果。本研究同时开展了一项试验，以测定采用标准野外调查方法或生物电阻抗分析法评估鱼类生理状态时的死亡率。本数据集配套的学术论文中详细记载了试验方法与研究结果。本数据集关联的期刊论文信息如下：Dibble, K.L.、Yard, M.D.、Ward, D.L. 及 Yackulic, C.B. 于2017年发表于《美国渔业学会汇刊》（Transactions of the American Fisheries Society）网络版的论文《生物电阻抗分析能否精准估算受胁与濒危沙漠鱼类的生理状态？》，DOI：10.1080/00028487.2017.1302993。