mTORC1 and mTORC2 associated proteins identified by 2D-LC-MS/MS

mTOR complexes were purified by immunoprecipitation (IP) with antibody directed against mTOR, Rictor or Raptor. Immunoprecipitates were analyzed by 2D LC-MS/MS. Proteins that were found in at least three out of four mTOR IPs, but not in mock IPs, were considered specific. IPs with antibodies directed against Rictor or Raptor indicated whether a candidate was specific for mTORC1 or mTORC2, respectively.