Tm and peak height values obtained for 20 different pathogens included in SeptiFast® test master list.

After SeptiFast analysis of the 20-microbial-target sample mix, we compared melting temperature (Tm) and peak heights for both extraction methods, SeptiFast® with MagNA pure compact extraction (MP), and SeptiFast® with manual extraction (SFM). In order to be considered valid, values had to be within accepted ranges of the SeptiFast identification software (SIS). Melting temperature (Tm) and peak heights are presented along with SIS interpretation of values obtained.