Zfp335 establishes eTreg lineage and neonatal immune tolerance by targeting Hadha-mediated fatty acid oxidation

We utilized single-cell RNA sequencing (scRNA-seq) to carry out comparative transcriptome analysis in Tregs from the spleens of WT and KO (Zfp335fl/flFoxp3-Cre). Overall design: High-dimensional single-cell RNA sequencing (10X Genomics) was used to profile the transcriptomes of YFP+ Tregs from the spleens of WT and KO (Zfp335fl/flFoxp3-Cre).