Mean values and standard deviations of Tlag, Trelease, ΔTrelease, Tlys, and ΔTlys values for individual germinating G. stearothermophilus spores*.

*Heat-activated G. stearothermophilus spores were germinated for 30 min with 1 mM L-valine or 1 mM AGFK at 65°C in 10 mM sodium phosphate buffer (pH 8.0), unactivated G. stearothermophilus spores were germinated at 65°C for 120 min with 60 mM CaDPA or with 1 mM dodecylamine in 10 mM sodium phosphate buffer (pH 8.0), and decoated G. stearothermophilus spores (heat-activated or unactivated) were germinated with different germinants at 65°C for 30 or 120 min. Kinetic parameters for individual germinations were determined by analysis of ≥100 spores that germinated as described in Methods.