Thomas A Planchon, Liang Gao, Daniel E Milkie, Michael W Davidson, James A Galbraith, Catherine G Galbraith, Eric Betzig (2011) CIL:36292, Sus scrofa domestica, epithelial cell. CIL. Dataset

Chromosome dynamics in a dividing LLC-PK1 cell. Images were collected with a Bessel Beam Microscope using two photon excitation. This form of microscopy provides extraordinary 4D resolution of the separation of chromosomes during division. This video corresponds to the left panel of CIL 34146, which shows views parallel and perpendicular to the mitotic plane. It also corresponds to Figure 5 and Supplementary Video 7 in Nat Methods. 2011 May;8(5):417-23. Epub 2011 Mar 4. The video is an deconvolved, maximum projection, volume rendering of slices that were collected in a 33 x 33 x 30 um3 image volume with 116 x 116 x 150 nm3 voxel sizes. The video has variable frame rates: frames 1-15 every 21 sec, frames 16-115 every 1.05 sec, frames 116-170 every 2 sec, frames 171-201 every 21 sec.

该数据集描述了在分裂的 LLC-PK1 细胞中染色体的动态变化。图像是通过贝塞尔光束显微镜利用双光子激发技术采集而成。此类显微镜提供了对染色体在分裂过程中分离的非凡四维分辨率。该视频对应于 CIL 34146 的左侧面板，展示了与有丝分裂平面平行和垂直的视角。此外，它还对应于 Nat Methods 2011 年 5 月第 8 卷第 5 期第 417-423 页的图 5 和补充视频 7。Epub 发布于 2011 年 3 月 4 日。视频是对切片进行去卷积、最大投影和体渲染的体积渲染，这些切片是在一个 33 x 33 x 30 立方微米的三维图像体积中，以 116 x 116 x 150 立方纳米的体素大小采集的。该视频具有变化的帧率：第 1-15 帧，每 21 秒一帧；第 16-115 帧，每 1.05 秒一帧；第 116-170 帧，每 2 秒一帧；第 171-201 帧，每 21 秒一帧。