Data from: Testing a “genes-to-ecosystems” approach to understanding aquatic-terrestrial linkages
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AbstractA ‘genes-to-ecosystems’ approach has been proposed as a novel avenue for integrating the consequences of intraspecific genetic variation with the underlying genetic architecture of a species in order to shed light on the relationships among hierarchies of ecological organization (genes [RIGHTWARDS ARROW] individuals [RIGHTWARDS ARROW] communities [RIGHTWARDS ARROW] ecosystems). However, attempts to identify genes with major effect on the structure of communities and/or ecosystem processes have been limited and a comprehensive test of this approach has yet to emerge. Here, we present an interdisciplinary field study that integrated a common garden containing different genotypes of a dominant, riparian tree, Populus trichocarpa, and aquatic mesocosms to determine how intraspecific variation in leaf litter alters both terrestrial and aquatic communities and ecosystem functioning. Moreover, we incorporate data from extensive trait screening and genome-wide association studies estimating the heritability and genes associated with litter characteristics. We found that tree genotypes varied considerably in the quality and production of leaf litter, which contributed to variation in phytoplankton abundances, as well as nutrient dynamics and light availability in aquatic mesocosms. These ‘after-life’ effects of litter from different genotypes were directly comparable to the responses of terrestrial communities associated with the living foliage. We found that multiple litter traits corresponding with aquatic community and ecosystem responses differed in their heritability. Moreover, the underlying genetic architecture of these traits was complex, and many genes contributed only a small portion to phenotypic variation. Our results provide further evidence that genetic variation is a key component of aquatic-terrestrial linkages, but challenges the ability to predict community or ecosystem responses based on the actions of one or a few genes., Usage notesCrutsinger et al.Excel format showing individual, community, and ecosystem data from mesocosm experiment sorted by the identity of the Populus trichocarpa genotype.Structural Equation Model R scriptmolecolLARS_SEM.rStructural Equation Model data filepop_dat.csv
摘要：“基因-生态系统（genes-to-ecosystems）”研究路径被提出为一种全新途径，旨在整合种内遗传变异的后果与物种的潜在遗传结构，以阐明生态组织层级（基因→个体→群落→生态系统）间的关联。然而，此前识别对群落结构和/或生态系统过程具有显著影响的基因的尝试仍受限于诸多瓶颈，且该研究路径的综合性检验尚未问世。本研究开展了一项跨学科野外研究，整合了包含优势河岸树种毛果杨（Populus trichocarpa）不同基因型的同质园实验与水生中宇宙（aquatic mesocosms）系统，以探究叶片凋落物的种内变异如何改变陆地与水生群落及生态系统功能。此外，本研究整合了大规模性状筛选与全基因组关联研究（genome-wide association studies）的数据，用以估算凋落物性状的遗传力及其关联基因。研究发现，不同杨树基因型的叶片凋落物质量与产量存在显著差异，这进而导致水生中宇宙中的浮游植物丰度、养分动态与光照可用性产生变异。不同基因型凋落物的这类后遗效应，与活体叶片关联的陆地群落响应具有直接可比性。本研究还发现，对应水生群落与生态系统响应的多项凋落物性状，其遗传力存在差异。此外，这些性状的潜在遗传结构较为复杂，多数基因仅对表型变异贡献微弱。本研究结果进一步证实遗传变异是陆地-水生联系的关键组成部分，但同时也挑战了基于单个或少数基因的作用预测群落或生态系统响应的可行性。 使用说明： Crutsinger等 本数据集为Excel格式，包含中宇宙实验的个体、群落与生态系统数据，按毛果杨基因型的标识排序。 结构方程模型R脚本：molecolLARS_SEM.r 结构方程模型数据文件：pop_dat.csv
