Additional file 9 of Extensive evaluation of ATAC-seq protocols for native or formaldehyde-fixed nuclei
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Additional file 9: Supplementary Table S9. List of ATAC-seq primers used for PCR and corresponding samples for each barcode. The Nextera N5 primer (Ad1_noMX) and the N7 primer “Ad2.1” and other N7 primer sequences (besides the barcodes) were obtained from [5]. Column 1 (“Name”) indicates the name we used for each primer. Column 2 (“Barcode in Fastq”) shows the sequence returned by the sequencer for that particular primer. Column 3 (“Oligo”) shows the sequence of the primers. Columns 4-11 specify the sample ID, buffer, transposition temperature, enzyme source, fixation state, nuclei input, replicate ID, and sample type for each sample indexed by the corresponding barcode.
附加文件9:补充表S9。用于PCR的ATAC-seq引物列表及其对应条码的样本。Nextera N5引物(Ad1_noMX)、N7引物“Ad2.1”以及其他N7引物序列(不包括条码)均来源于[5]。第1列(“名称”)表示我们对每个引物所使用的名称。第2列(“Fastq中的条码”)显示了测序仪为该特定引物返回的序列。第3列(“寡核苷酸”)显示了引物的序列。第4至11列分别指定了由对应条码索引的每个样本的样本ID、缓冲液、转座温度、酶源、固定状态、核输入、重复ID和样本类型。
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