DNA methylation dynamics in primary human monocytes exposed to thyroid hormone ex vivo. DNA methylation dynamics in primary human monocytes exposed to thyroid hormone ex vivo

Trained immunity is a phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to certain microbial components, altering their responses to future exposures. This study profiled the genome-wide DNA methylation profile of human monocytes exposed to thyroid hormone for 24 hours. Overall design: Human primary monocytes were isolated using Ficoll and two rounds of Percoll gradient separation. Baseline monocytes were collected after 1 hour in culture (attached) and 24 hours samples were collected for media only, LPS only, Thyroid hormone (T3), or T3+LPS.