Single cell RNA-seq of 4T1-T vasculogenic mimicry-competent mammary tumors

We have previously described a sub-clones of the 4T1 mammary carcinoma cell line that are proficient for vasculogenic mimciry (VM), namely 4T1-E and 4T1-T. In vitro assays suggest that not all cells within these lines a VM-competent. To explore subsets of cells within tumors derived from these cells that may display VM properties we utilized single cell RNA-Seq of 4T1-T mammary fat-pad tumors. Overall design: 50,000 Parental 4T1-T cells labelled with CellTag vector were implanted into the mammary fat-pad of 6-8 week old female BALB/c mice. At day 20 post-implantation tumors were dissociated and submitted for single cell RNA-seq to the genomics core facility at the CRUK Cambridge Institute.