RNA demethylase ALKBH5 promotes tumorigenesis in multiple myeloma [RNA-seq]. RNA demethylase ALKBH5 promotes tumorigenesis in multiple myeloma [RNA-seq]

We here report ALKBH5, a m6A RNA demethylase, as a crucial oncogene in multiple myeloma (MM). Using various MM models, we demonstrated a critical requirement of ALKBH5 for MM cell proliferation in vitro and in vivo. RNA-seq was applied to compare the expression profile of MM cells with or without ALKBh5 knockdown. Overall design: RNA-seq of RPMI-8226 and CAG cells with or without shRNA-mediated ALKBH5 konckdown