RNA-sequencing data of Balb/c involuting mammary gland treated with anti-Ly6G antibody and estrogen

Female Balb/c mice were oxvariectomed at lactation day 2, pups forced weaned at lactation day 12 to initiate mammary involution. At 24 hours post-weaning, mice were treated with anti-Ly6G antibodies or isotype control. At 48 hours post-weaning, mice were treated with 20µg/kg of body weight estrogen or vehicle control. Mice sacrificed after 24 hours estrogen treatment and mammary sample collected and frozen. Mammary samples were extracted for total RNA using Trizol reagen and then treated with Dnase1 before sending for library preparation and sequencing by A*STAR GIS using illumina HiSeq4000.