Transcriptomic comparative analysis of uninfected and L. amazonensis-infected Bone Marrow-Derived Dendritic Cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE144039
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资源简介：
To determine the modulation of gene expression of mouse BMDCs in the presence of living intracellular Leishmania amazonensis amastigotes at 24 hr post infection. Dendritic cells were generated in vitro from bone marrow progenitors. Leishmania amazonensis amastigotes were purified from mouse cutaneous lesions and were added to DC cultures. After 24h, and following a sorting procedure using the FacsAria, only BMDCs housing living amastigotes were selected for total RNA extraction based on the expression of MHC class II (host cell) and DsRed2 (parasites) molecules. Transcriptome profiling was carried out using a one-color hybridization setting on Affymetrix GeneChip mouse genome 430 2.0 arrays.
创建时间：
2020-06-25
