LIF-responsive genes in tumor-associated myeloid cells from ascitic fluid of syngeneic ovarian cancer model. Mus musculus

LIF has an important role in immunosupression in different scenarios, such as embryo implantation in the uterus, autoimmune disease or organ transplantation. We decided to study the role of LIF on the immune response to cancer. We found that tumors expressing high levels of LIF promote an immune-tolerant microenvironment precluding the anti-tumor immune response. To establish the effect of LIF on tumor-associated myeloid cells (TAMCs), we used a syngeneic ovarian tumor model (ID8 cell line). We treated animals bearing tumors with anti-LIF antibody and isolated myeloid (CD11b+) cells from the ascites. RNA was extracted from the CD11b+ cells and the transcriptomic analysis was performed. Overall design: Tumor bearing C57BL6 mice were treated from the day 12 after inoculation with anti-LIF neutralizing antibody for 25 days (twice a week)and euthanized. The ascitic fluid was extracted and myeloid cells were isolated with CD11b magnetic beads. CD11b+ cells were lysed for RNA extraction and hybridized on Affymetrix microarrays. We sought to obtain a decrease in M2-like phenotype genes in response to the treatment.