Dataset for 'Development of an Infection-Responsive Fluorescent Sensor for the Early Detection of Urinary Catheter Blockage'

Formation of crystalline biofilms following infection by Proteus mirabilis can lead to encrustation and blockage of long-term indwelling catheters, with serious clinical consequences. We describe a simple sensor, placed within the catheter drainage bag, to alert of impending blockage via a urinary colour change.

The dataset presented shows data from the characterisation and testing of the prototype sensors, Including: investigation into the fluorescent properties of 5(6)-carboxyfluorescein (CF) (fluorescence output vs CF concentration and fluorescence output vs solution pH), change in pH vs time for P. mirabilis subcultures and bacterial supernatants, corresponding dye release vs time, as well as data from the testing of the sensors within the in vitro bladder model system (change in fluorescence output within the drainage bag and approximate CF concentration with time). Investigation into the initial dye release kinetics was also undertaken and the data presented in this dataset. Standard curves at three different pHs (6,7 and 8) are presented as well as the overlaid release profiles over time.

革兰氏阴性菌奇异变形菌（Proteus mirabilis）感染所引发的晶体生物膜形成，可能导致长期留置导尿管的沉积和堵塞，进而产生严重的临床后果。本研究描述了一种简单的传感器，该传感器置于导尿管引流袋中，通过尿液颜色的变化来预警即将发生的堵塞。所提供的数据集展示了原型传感器在特征化和测试过程中的数据，包括：对5(6)-羧基荧光素（CF）的荧光特性研究（荧光输出与CF浓度及荧光输出与溶液pH值的关系）、奇异变形菌子代培养物和细菌上清液pH值随时间的变化、相应的染料释放与时间的关系，以及传感器在体外膀胱模型系统中的测试数据（引流袋内荧光输出的变化及随时间变化的CF浓度估计）。此外，还对初始染料释放动力学进行了研究，并将相关数据包含在本数据集中。同时展示了三种不同pH值（6、7和8）的标准曲线，以及随时间叠加的释放图谱。