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Bulk RNA-seq for undifferentiated spermatogonia from the testes of control, aged and busulfan-treated C57BL/6 mice

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Samples were prepared from 100 GFRa1-GFP positive, Miwi2-tdTom negative and 100 GFRa1-GFP negative, Miwi2-tdTom positive cells that were sorted with FACS. cDNA libraries were prepared with the SMARTseq2 protocol. Adult, control samples had four biological replicates while the aged and busulfan-treated samples were in triplicate. There were 2 technical replicates per biological sample.

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