Expression data of the kidney tissues from wildtype and Tet2 KO mice

Our previous study has showed Ten-eleven translocation (Tet2) gene expression as well as the global 5-hydroxymethylcytosine (5hmC) level were reduced significantly in mouse kidney insulted by ischemia reperfusion (IR). Here, we investigate the functional role of Tet2 in renal IR injury. We constructed renal IR injury model in wild type and Tet2 knockout (KO) mice. Serum creatinine (SCr) and blood urea nitrogen (BUN) were examined to evaluate renal function. HE staining and renal injury biomarkers were tested to confirm the degree of injury. Microarray analysis of mRNA expression levels was carried out to gain mechanistic insightinto the role of Tet2. We used microarrays to profile the transcriptomes of the kidneys from wildtype and Tet2 KO mice. Wildtype and Tet2 KO mice were subjected to sham surgery or renal ischemia reperfusion. The kidney tissues of these mice were collected for RNA extraction and microarray analysis.