Regulation Of Satiety and Epigenetic mechanisms in pigs. ROSEpigs

Characterization of the transcriptome and methylome of 2 divergent porcine lines selected on a feed efficiency criterion (RFI, Mean Residual Daily Intake), with a poorly efficient line (RFI+) and an efficient line (RFI-) presenting different feeding behaviors. The aim is to compare the transcriptome of the duodenum mucosa of these two lines: i) on an empty stomach, to compare the gene expression levels of these two lines with each other, and ii) after a meal, to compare the transcriptome response to the passage of the alimentary bolus in each of the lines.The duodenal DNA methylmome was characterized in these same samples by DNA methylation precipitation followed by high throughput sequencing to estimate the proportion of the observed transcriptomic differences that is associated with changes in DNA methylation.