MsMYB62-like as a negative regulator of anthocyanin biosynthesis in <i>Malus spectabilis</i>

Crabapple is a valuable tree species in gardens due to its captivating array of flower and leaf colors, rendering it a favored choice in landscaping. The economic and ornamental values of <i>Malus</i> crabapple are closely associated with the biosynthesis of anthocyanin, a pigment responsible for its vibrant hues. The intricate regulation of anthocyanin biosynthesis involves the concerted activity of various genes. However, the specific mechanism governing this process in crabapple warrants in-depth exploration. In this study, we explored the inhibitory role of MsMYB62-like in anthocyanin biosynthesis. We identified <i>MsDFR</i> and <i>MsANS</i> as two downstream target genes of MsMYB62-like. These genes encode enzymes integral to the anthocyanin biosynthetic pathway. The findings demonstrate that MsMYB62-like directly binds to the promoters of <i>MsDFR</i> and <i>MsANS</i>, resulting in the downregulation of their expression levels. Additionally, our observations indicate that the plant hormone cytokinins exert a suppressive effect on the expression levels of <i>MsMYB62-like</i>, while concurrently upregulating <i>MsDFR</i> and <i>MsANS</i>. This study reveals that the MsMYB62-like-<i>MsDFR</i>/<i>MsANS</i> module plays an important role in governing anthocyanin levels in <i>Malus</i> crabapple. Notably, the regulatory interplay is modulated by the plant hormone cytokinins.