YB1 dephosphorylation attenuates atherosclerosis by promoting CCL2 mRNA decay

Y-box binding protein 1 (YB1) is an RNA binding protein (RBP) that has been reported to play important roles in inflammation and atherosclerotic plaque formation. To explore the molecular mechanism of phosphorylated YB1 (pYB1) in atherosclerosis in vitro, we constructed YB1 phosphorylation site (Ser-100) mutant stable smooth muscle cell line (3ds-V5) and performed RNA profile screening through RNA-seq.  We found inflammatory pathways and CCL2 was significantly decreased in the 3dS-V5 YB1 mutant compared with the control group (YB1-V5).