MudPIT analyses of the proteins associated with Kelch-like protein 6 (KLHL6) in a human multiple myeloma cell line, ARP-1, and in HEK293T cells

Kelch-like protein 6 (KLHL6) is a highly conserved and uncharacterized BTB-Kelch protein with a lymphoid tissue-restricted expression pattern. To gain insight into the molecular function of KLHL6 and to understand the impact of the cancer-associated mutations, we compared the protein interactome of KLHL6(WT) to that of the cancer mutant KLHL6(L65P), a BTB-domain mutant frequently mutated in B-cell cancers. FLAG-KLHL6(WT) or FLAG-KLHL6(L65P) complexes were biochemically immunopurified from two different cell lines (HEK293T and ARP-1).