Figure 1. Bifidobacterium alters DC and Macrophage surface phenotype.

<strong>Figure 1.</strong> <em><strong>Bifidobacterium</strong></em><strong> alters DC and MF surface phenotype. </strong>DC and MF cultured with media alone (to which treatment groups are normalized), ATCC25526 (ATCC) or UMB-MBP-01 (MD) UV-killed <em>Bifidobacterium</em> or EPS derived from each strain. After 24 hrs of culture, cells analyzed by flow cytometry. DC gated on live CD11c+, and MF gated on live F4/80+ populations. DC stained for <strong>A) </strong>MHC class II, <strong>B) </strong>CD40, <strong>C) </strong>CD80, and <strong>D) </strong>CD86. MF stained for <strong>E) </strong>MHC class II, <strong>F) </strong>CD40 and<strong> G) </strong>CD80. MFI: normalized mean fluorescence intensity; MFI values normalized to control and compared using one-way ANOVA. * p value &lt; 0.05; ** p value &lt; 0.01. UV-killed MF data representative of 3 separate experiments (one of which is shown), 2 wells/culture condition, i.e. 6 total wells per condition over 3 experiments. EPS MF data representative of 2 separate experiments (one of which is shown), 2 wells/culture condition, i.e. 4 total wells per condition over 2 experiments. DC data merged from one experiment with EPS treatment and 2 experiments with UV-killed bacteria (one of which is shown), each data set is normalized to its respective “media only” control, i.e. 4 total wells per condition over 2 experiments for UV-killed bacteria and 2 total wells per condition for EPS.