Data from: Colonial choanoflagellate isolated from Mono Lake harbors a bacterial community

As the closest living relatives of animals, choanoflagellates may offer insights into the ancestry of interactions among animals and bacteria. Here we report the isolation and characterization of a colonial choanoflagellate species, <em>Barroeca monosierra</em> harbors a bacterial community in its lumen. <em>B. monosierra</em> was isolated from Mono Lake, California and forms large spherical colonies that are an order of magnitude larger than those formed by the closely related choanoflagellate <em>Salpingoeca rosetta</em>. The rosettes, which can develop from single cells, are filled with a branched network of undefined extracellular matrix. We found that lab-reared <em>B. monosierra</em> colonies are colonized by members of the halotolerant Saccharospirillaceae and Oceanospirillaceae, as well as purple sulfur bacteria (Ectothiorhodospiraceae) and non-sulfur Rhodobacteraceae. This relatively simple bacterial community in a close relative of animals presents a new experimental model for investigating the evolution of stable interactions among eukaryotes and bacteria.
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This dataset contains the genome sequence of <em>B. monosierra</em>, as well as 24 associated bacterial genome sequences.<br>

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The two ZIP archives whose names begin with "tree" contain all relevant data from the phylogenetic trees presented in Figure 1C and Figure S1. Each archive contains the following subdirectories:
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input: input sequences in FASTA format
polyA_removed: polyA tails removed by trimest (for protein coding genes only)
aligned: sequence alignments by FSA
trimmed: trimmed alignments by trimAl
RAxML: input, run parameters and output of RAxML maximum likelihood tree reconstruction
MrBayes: input, run parameters and output of MrBayes Bayesian tree reconstruction
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The file Figure_2E.tree contains the tree from Figure 2E in Newick format.<br>