Gene expression profiles of tumor-induced pTregs and anergic tumor-specific CD4+ T cells

Up to now the role of tumor-specific pTregs and anergic cells during tumor development is not fully understood. Here we used a genetically-induced tumor expressing a MHC-II restricted DBY model antigen to characterize the tumor-induced pTregs and anergic cells that arise early during tumor development. Naïve DBY-specific Marilyn CD4 T cells were transferred into mice bearing DBY expressing tumors at different stages of tumor growth. Marilyn pTregs or anergic Marilyn cells were purified 7 days after transfer from the lymph node draining the tumor site.