RNA-seq of the lacrimal gland mesenchymal tissue in control vs Shp2-deleted neural crest mouse embryos

The goal of the study is to find the transcriptional targets downstream of Shp2-mediated signaling regulating Fgf10 production in the lacrimal gland mesenchyme. In order to look for the potential targets, we performed laser capture microdissection of the lacrimal gland mesenchyme tissue at embryonic day E14.5 from control embryos as well as mutant embryos containing neural crest-specific deletion of Shp2. After tissue harvest, RNA was extracted and conversion to cDNA as well as amplification was performed by NUGEN’s ovation kit v2. After preparation of cDNA library, each sample was sequenced using Illumina platform. For this experiment, one control sample and one mutant sample were analysed.