Age-related TFEB Downregulation in Proximal Tubules Causes Systemic Metabolic Disorders with Occasional ApolipoproteinA4-related Amyloidosis

We generated proximal tubular epithelial cell-specific Tfeb-deficient mice and bred them for up to 24 months and found that Tfeb deficiency in the proximal tubules causes metabolic disorders, which occasionally lead to apolipoproteinA4 (APOA4) amyloidosis, with age. To elucidate the mechanism of the metabolic disturbances, we performed scRNA-seq analyses of the kidneys. Renal cortex samples dissected from kidneys were isolated into single cells and analyzed using scRNA-seq.