Repression of germline genes in C. elegans somatic tissues by H3K9 dimethylation of their promoters [ChIP-seq]

synMuv B proteins, including LIN-15B, are necessary to repress the expression of germline-promoting genes in the soma during embryonic and early larval development in C. elegans. The goal of this research was to determine if changes to histone modifications could underlie the misexpression of germline genes in these mutants. We performed ChIP-seq on four histone modifications in wild type and three synMuv B mutants at two temperatures to assess these changes. Examination of four histone modifications (H3K9me2, H3K27me3, H3K4me3, H3K36me3) by ChIP in WT and three mutant (lin-15B(n744), lin-35(n745), lin-37(n758)) C. elegans at 20°C and 26°C to assess changes in chromatin that may lead to the changes in gene expression seen in mutants.