RNA-Sequencing of rats treated with Chinese herbal medicine Pulsatilla Chinensis

Pulsatilla chinensis (PC) is a traditional Chinese medicinewith detoxification activities. It has been used for dysentery, vaginal trichomoniasis, bacterial infections and malignant tumor treatment. Gavage administration of PC in hyperlipidemia rats for 11 weeks resulted in obviously reduced serum total cholesterol, LDL-cholesterol and ameliorated fatty liver. So we wanted to further investigate the relationship between PC and lipid metabolism related pathways or genes Analysis used normal diet treated rats as control samples for comparison to the model samples treated with high cholesterol-high fat diet ,and the treatment group with high cholesterol-high fat diet plus Pulsatilla Chinensis for comparison to the model samples treated with high cholesterol-high fat diet , genes expression changes were determined by RNA-Seq to determine Pulsatilla Chinensis responsive genes