RNA-seq analysis of Sphingosine 18:1 treated mice bone marrow-derived macrophages
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE262135
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Purpose: Study of the influence of Sphingosine 18:1 in macrophages Method: 2μM Sphingosine 18:1 was added to BMDMs for 6 hours, solvent was added as control. mRNA profiles were generated by deep sequencing. Results: Several signaling pathways were changed after Sphingosine administration, including hypoxia-related signal pathway. RT-quantitive PCR was performed to validate the expression of Epas1 and its downstream target genes, results showed that Sphingosine 18:1 significantly inhibits Epas1 expression. Expression profiling by RNA-seq, profiling of BMDMs after Sphingosine administration.
创建时间：
2024-06-25
