Identification of KLF4 regulated transcriptional features in breast cancer

Kruppel-like factor 4 (KLF4), as a eukaryotic transcription factor, participates in the regulation of cell proliferation, differentiation, embryonic development and other important life processes. KLF4 is also closely related to the occurrence and development in variety types of tumor. To determine the functions of KLF4 in breast cancer, we over-expressed KLF4 in breast cancer LM2-4175 cells and used gene expression profiling to identify KLF4 regulated genes. The stable KLF4 over-expressed LM2-4175 cell lines were generated by transduction a lentiviral-mediated expression plasmid. Total RNA from LM2-4175 control cells and KLF4 over-expressed LM2-4175 cells was collected and profiled to Affymetrix microarray.