Accumulation of HT-2 toxin from contaminated mushroom compost by edible <i>Agaricus bisporus</i>

Wheat straw is commonly used as a cellulose source in mushroom compost and could be a secondary source of mycotoxin contamination in the food chain. We cultivated edible <i>Agaricus bisporus</i> and <i>Pleurotus ostreatus</i> on T-2/HT-2 artificially-contaminated mushroom compost and developed and in-house validated an UHPLC-MS/MS method for determination of T-2, HT-2, T2-triol and T2-tetraol in mushroom compost and mushroom basidiocarp. A rapid phase I metabolization of T-2 and HT-2 in mushroom compost was observed. In <i>Agaricus bisporus</i>, basidiocarps 8–15 µg kg⁻¹ accumulation of HT-2 calculated on wet weight was measured. No detectable mycotoxins were found in <i>Pleurotus ostreatus</i> basidiocarp.小麦秸秆常被用作蘑菇堆肥的纤维素来源，同时也可能成为食物链中真菌毒素污染的次生来源。本研究将食用双孢蘑菇（Agaricus bisporus）和平菇（Pleurotus ostreatus）接种于经T-2、HT-2人工污染的蘑菇堆肥中，建立并通过实验室内部验证了一种用于检测蘑菇堆肥及食用菌担子果中T-2、HT-2、T2-三醇与T2-四醇的超高效液相色谱-串联质谱（UHPLC-MS/MS）方法。研究观察到T-2与HT-2在蘑菇堆肥中发生了快速的I相代谢。在双孢蘑菇中，其担子果内HT-2的积累量为8~15 μg·kg⁻¹（以湿重计）。平菇担子果中未检出任何可检测水平的真菌毒素。

搜集汇总

背景与挑战

背景概述

该数据集研究双孢蘑菇从受污染的蘑菇堆肥中积累HT-2毒素的情况。研究通过人工污染堆肥培养蘑菇，并采用UHPLC-MS/MS方法检测毒素，发现双孢蘑菇子实体中HT-2毒素积累量为8-15 µg kg−1（湿重），而平菇中未检测到毒素。