Role of interstitial cells of Cajal in regulating tone and responses to enteric motor neurons in the murine pyloric sphincter

Gastric slow waves fail to propagate through the pyloric sphincter (PS), thus isolating the specialized motility patterns of the stomach and small intestine. We investigated the role of interstitial cells of Cajal (ICC) in PS of mice.  Ca2+ waves in ICC, events responsible for electrical slow waves, propagated along the gastric wall but failed to propagate into the PS.  ICC in PS fired localized Ca2+ transients and displayed low expression of voltage-dependent Ca2+ conductances.  These are properties of intramuscular ICC (ICC-IM) that cannot regenerate and propagate slow waves.  A T-type Ca2+ channel antagonist had no effect on Ca2+ transients, but these events were blocked by thapsigargin and CPA, suggesting they result from Ca2+ release.  PS ICC expressed ANO1, a Ca2+-activated Cl- conductance.  Ca2+ released from stores actives ANO1 channels, thus exerting a depolarizing influence on PS.  Ani9, selective antagonist of ANO1 channels, hyperpolarized c..., , # Role of interstitial cells of Cajal in regulating tone and responses to enteric motor neurons in the murine pyloric sphincter

Dataset DOI: [10.5061/dryad.7wm37pw71](https://doi.org/10.5061/dryad.7wm37pw71)

## PS & ICC Ca²⁺ Imaging and Electrophysiology Dataset

This dataset contains summary data analyses of calcium imaging, gene expression, and electrophysiological experiments in interstitial cells of Cajal (ICC) and pyloric sphincter (PS) muscles in mice. All data files are provided as GraphPad Prism (.prism) files. Supplemental movies illustrating calcium imaging experiments are also included.

### Prism Data Files

Fig_1ICC_Ca2__imaging_in_the_whole_murine_stomach._Calcium_waves_freq_and_propagation.prism

Calcium waves across the whole stomach (corpus to antrum) in ICC GCaMP mice. ST maps were generated using Fiji/ImageJ; duration and propagation velocity of each wave were measured and analyzed with GraphPad Prism 10.

Fig_2-ICC_Ca2__signals_pyloric_sphincter._Frequency_and_dur...,