Native MS dataset for: Mechanistic insights into ssDNA recognition and recombination by bacterial RAYT proteins.

Native mass spectrometry dataset used in: Mechanistic insights into ssDNA recognition and recombination by bacterial RAYT proteins. Iva Nečasová, Jaroslav Nunvář, Jakub Svoboda, Táňa Černovec, Alan Kádek, Prokopis C. Andrikopoulos, Bohdan Schneider, Jiří Zahradník. Journal XX (2026) Samples from affinity purification eluted in the presence of 1mM EDTA were buffer exchanged by two consecutive passes over centrifugal gel filtration columns (Micro Bio-Spin P-6, 6-kDa cut off; BioRad) into 250 mM ammonium acetate pH 8.0 acting as MS-compatible buffer surrogate. Samples diluted at 10 µM were loaded into in-house prepared golden-coated borosilicate glass capillaries (1B120F-4, World Precision Instruments) and electrosprayed into a Waters Synapt G2Si mass spectrometer. The mass spectrometer was operated in positive sensitivity ion mode over the mass range of 500 – 230,000 m/z and tuned to achieve low ion activation with maximal signal intensity. The ESI capillary and sampling cone were kept at 1.0 kV and 40 V, respectively, source temperature 80°C. Argon was used as the collision gas at 6 ml/min flow. Data were acquired with variable trap collision energy voltage of 10V and 40V. For top-down MS/MS analysis, precursor peak at m/z 2439 was isolated in a quadrupole and subjected to CID fragmentation by step-wise ramp of the trap collision voltage to 90V. DAcquired data were smoothed and externally mass recalibrated on caesium iodide clusters in MassLynx 4.2 (Waters) with mass deconvolution in UniDec 6.0.48.1.1 (10.1021/acs.analchem.5b00140). Description of individual files' contents as well as parameter changes during the acquisition are described in the accompanying text file.