CUT&RUN analysis for phospho-MYB and active histone marks in FKBP-MYB Jurkat cells.
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE267360
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CUT&RUN analysis was performed in Jurkat cells with FKBP knock-in at the MYB locus to analyze DNA bindings of phospho-MYB, H3K27ac, H3K4me3 after dTAG-mediated depletion. Jurkat cells were incubated with CUTANA pA/G-Mnase. Chromtin fragments were purified utilizing a Cutana DNA purification kit. Libraries were generated using a NEBNext Ultra II DNA library prep kit and analyzed on Illumina NovaSeq at GENEWIZ Azenta Life Sciences Co. Ltd.
创建时间：
2024-11-11
