Table 1 in Systematics of the Neotropical genus Catharylla Zeller (Lepidoptera, Pyralidae s. l., Crambinae)

Table 1. PCR mix for respective genes and primer pairs. ProductLCO/Pat, LCO/Nancy, Jerry/ Pat (COI) HybLepWg1 / HybLepWg2 (wingless)LCO/K699, Ron/Nancy, Jerry/Mila, Brian/Pat (COI), HybLepWg1/ HybLepWg2 (wingless), Oscar-6143 /Bosie-6144 (EF-1α)HybLepWg1/HybLepWg2 (wingless), Oscar-6143/ Bosie-6144 (EF-1α)H2O (ultraclean)15.9 μl12.55 μl11.95 μl10X Taq buffer (15 mM MgCl2)2.5 μl2.5 μl2.5 μl (10X OptiBuffer)MgCl2 (25 mM)1 μl0.75 μl0.75 μl (50 mM)Forward primer (10 pmol/μl)0.5 μl1.2 μl1.5 μlReverse primer (10 pmol/μl)0.5 μl1.2 μl1.5 μldNTP (each 10 mM)0.5 μl2.5 μl2.5 μlTaq DNA polymerase (5 units/μl)0.1 μl0.3 μl0.3 μl (BIO-X-ACT Short TaqDNA polymerase; 4 units/μl)DNA4 μl4 μl4 μl