Alligator induced Pluripotent Stem Cell Oil/Dispersant Toxicity Test

Alligator induced Pluripotent Stem Cells (iPSCs) were treated with various concentrations of oil/dispersant mixture (MC252 source oil/dioctylsodium sulfosuccinate [DOSS], at constant ratio of MC252:Doss of 1:10) to assess viability of cells after 48 hour exposure. Viable cells were labeled with sulforhodamine B (SRB) and absorbance was quantitated using the Typhoon scanner. There was a trend toward increasing cell viability with increasing concentrations, with maximum viability observed at 100ppm oil/dispersant.