A selection of differentially expressed genes from microarray expression studies.

The genes listed are either downregulated or upregulated in the N. meningitidis MC58 modA11::kan mutant, N. meningitidis B6616/77 modA12::kan mutant, N. gonorrhoeae 96D551 modA12::kan mutant, or N. gonorrhoeae FA1090 modA13::kan mutant (refer to Table S3, Table S4, Table S5, and Table S6 for a complete list of downregulated or upregulated genes in the N. meningitidis modA11 and modA12 mutants and N. gonorrhoeae modA12 and modA13 mutants). The identity of the gene is indicated with the gene ID in the annotation of the N. meningitidis strain MC58 genome, N. meningitidis strain Z2491 genome, or N. gonorrhoeae strain FA1090 genome (TIGR). The average ratio presented is the mean of N. meningitidis MC58 modA11::kan mutant: wild-type MC58 modA11 ON and N. meningitidis B6116/77 modA12::kan mutant: wild-type B6116/77 modA12 ON, or the mean of N. gonorrhoeae 96D551 modA12::kan mutant: wild-type 96D551 modA12 ON and N. gonorrhoeae FA1090 modA13::kan mutant: wild-type FA1090 modA13 ON, from six replicate spots on three independent microarrays, incorporating a dye swap. Only those genes with an expression ratio above 1.5-fold were included in this study.*Confirmed by Western blot (Figure 4C).?Confirmed by ��-galactosidase assay (see Figure S3 and Figure 4B).��Quantitative RT-PCR (qRT-PCR) was also done with N. gonorrhoeae strain O1G1370 wild-type modA13 ON and O1G1370 modA13::kan mutant. Results are as follows: NGO1581; 0.524��0.101, mtrF; 4.59��0.264, metF; 3.51��0.805. Gene expression confirmed by qRT-PCR in a natural FA1090 modA13 ON and FA1090 modA13 OFF strain. Results are as follows: NGO1581; 0.090��0.101, mtrF; 17.11��0.956, metF; 3.51��0.477.