16s SRA data of breast milk flora and stool flora of eczema infants

Imbalanced development of immune system during early life undermines infants\u2019 growth and wellbeing. Infantile eczema, usually coupled with a range of hypersensitive phenotypes, has come into notice with its rising prevalence and unclear pathogenesis. According to recent studies, close ties have been discovered between eczema and infants\u2019 intestinal flora. To gain a further understanding toward interactions between microbiota and eczema, we involved breast milk flora as a new factor and presented the links among breast milk flora, infant intestinal flora and infantile eczema by a cohort study in northeast China. Totally 52 families were recruited with either eczema infant or healthy infant under 6-month-old in this study. Analysis and predictions were made based on amplicon sequencing of microbiota. As results, Bifidobacterium and Bacteroidetes were found enriching in healthy infant stools and eczema infant stools separately which was consistent with previous reports. Besides, distinct compositional features were observed in breast milk flora simultaneously as more \u201cpositive\u201c bacteria such as Akkermansia, certified with immunity homeostasis improvement, was enriched in breast milk from healthy infant\u2019s mothers. On the bacteria delivery part, higher bacteria delivery efficiency was found in pairs of breast milk flora and stool flora in families with eczema infant compared with families with healthy infant. Bacteroidetes, a widely known indicator of eczema was also found delivered from breast milk in eczema pairs as well. Further metagenomic predictions revealed that breast milk microbiota was found significantly lower presented in metabolism and immune system pathway, especially in antigen processing and presentation and Th17 cell related pathways. In conclusion, as other components of breast milk, breast milk microbiota also closely connects with infant health via mother-infant bacteria delivery and metabolic functions of itself.