SMRT sequencing of full-length transcriptome of wild silkworm, Bombyx mandarina

In order to characterize the transcriptome of the Bombyx mandarina and further increase genomic resource, we sequenced the mRNA of full-length complementary DNA (cDNA) libraries derived from pooled tissues of wild silkworm by employing the single-molecular real-time sequencing (SMRT). Tissues of different developmental stages were mixed into two sample pools, multiple size-fractionated libraries including 1-2, 2-3 and 3-6kb library were instructed and sequenced on the PacBio Sequal platform. For the two sequencing pools, 11.55Gb and 11.80Gb of clean reads was obtained respectively. After merge the clean reads and remove redundancies, we got 93 616 non-redundant transcripts at last. The availability of whole transcriptome database and their isoform information will be useful to improve our understanding of the domestic mechanism, and also for the analysis of development dynamics in the Bombyx mandarina.