Temperature effects on dissemination probability of Ae. aegypti infected with DENV-1 exposed to low temperatures.

At each time point, the effect of temperature, and carton nested within temperature was analyzed for the 26°C and 20°C constant temperatures, and the 20°C cyclic treatment. 16°C constant was not included in tests because only single individuals with a detectable infection at 7 and 21 days post-exposure were identified, neither of which had a disseminated infection.