CIL:12288, Homo sapiens, epithelial cell. In Cell Image Library
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MCF-10A epithelial cells were fixed with 1:1 mixture of methanol/acetone (-20° C, 10 min), permeabilized with 0.5% Triton in PBS, and immunostained sequentially with primary, then secondary antibodies and (Cy5, 633 nm laser), laminin-5 (Cy2, 488 nm laser) and integrin a3 (rhodamine red-X, 543 nm laser), and observed using a confocal laser-scanning microscope system. For additional details see: J. Cell Biol. 2010. 189(5):901-917
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UC San Diego Library Digital Collections
创建时间：
2021-04-15
