The transcriptomic and proteomic changes of <i>Hannaella sinensis</i> in response to patulin stress

Patulin (PAT) is a significant mycotoxin contaminant of fruit and fruit products. <i>Hannaella sinensis</i> is an antagonistic yeast that controls postharvest diseases and mycotoxins in crops and is potentially valuable for ensuring food safety. Our previous studies have shown that <i>H. sinensis</i> can effectively degrade PAT. The possible physiological mechanisms of yeast have been previously reported, however, the molecular mechanism of PAT degradation by the yeast needs to be explored in depth. Therefore, our current work aimed to use transcriptomic and proteomic techniques to unravel the molecular mechanisms by which PAT degradation occurs in <i>H. sinensis</i>. The results showed that transcriptomic studies of <i>H. sinensis</i> in PAT culture identified 951 differentially expressed genes, of which 194 were up-regulated, and 757 were down-regulated. These genes could be used to induce the ability of <i>H. sinensis</i> to resist stress, promote the synthesis of drug-resistant substances and maintain the integrity of cell membranes. Besides, proteomic analyses revealed that PAT treatment enhances multiple aspects of <i>H. sinensis</i>, including protein processing, cell growth, reproduction, catalase, cell wall synthesis, and more. Our study helps to understand the possible mechanisms of PAT degradation and PAT stress response at the transcription and protein expression levels in <i>H. sinensis.</i>

展青霉素（Patulin, PAT）是一类严重污染水果及果蔬制品的真菌毒素。中华汉拿酵母（Hannaella sinensis）是一类可防治作物采后病害与真菌毒素污染的拮抗酵母菌，在保障食品安全方面具有潜在应用价值。既往研究表明，该酵母可有效降解展青霉素。此前虽已有关于该酵母生理降解机制的相关报道，但该酵母降解展青霉素的分子机制仍有待深入探究。因此，本研究旨在通过转录组学与蛋白质组学技术，解析中华汉拿酵母降解展青霉素的分子机制。研究结果显示，对在展青霉素培养基中培养的中华汉拿酵母进行转录组分析，共鉴定得到951个差异表达基因，其中194个基因上调表达，757个基因下调表达。这些基因可参与诱导酵母的抗逆能力、促进抗药性物质合成以及维持细胞膜完整性。此外，蛋白质组学分析发现，展青霉素处理可从多个层面增强中华汉拿酵母的相关功能，包括蛋白质加工、细胞生长、繁殖、过氧化氢酶活性、细胞壁合成等。本研究有助于从转录与蛋白质表达层面解析中华汉拿酵母降解展青霉素以及应对展青霉素胁迫的潜在机制。

搜集汇总

背景与挑战

背景概述

该数据集研究了拮抗酵母Hannaella sinensis对霉菌毒素patulin（PAT）应激的转录组和蛋白质组变化。通过分析发现，PAT处理导致951个差异表达基因（194个上调，757个下调），这些基因涉及应激抵抗、耐药物质合成和细胞膜完整性；同时，蛋白质组学显示PAT增强了蛋白质加工、细胞生长、繁殖和细胞壁合成等过程。这有助于从分子水平理解H. sinensis降解PAT和应对应激的潜在机制。